{"id":2505,"date":"2023-04-07T16:50:00","date_gmt":"2023-04-07T16:50:00","guid":{"rendered":"http:\/\/livinglight.icfo.eu\/?p=2505"},"modified":"2023-04-07T16:53:35","modified_gmt":"2023-04-07T16:53:35","slug":"flintstones-safe-harbor-integration-of-transgenes-using-crispr","status":"publish","type":"post","link":"https:\/\/livinglight.icfo.eu\/?p=2505","title":{"rendered":"FLINTstones &#8211; safe harbor integration of transgenes using CRISPR"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">Happy to announce that Nawaphat&#8217;s paper is peer reviewed and proofed. If you have checked the preprint already, check this one put again &#8211; it has some minor additions. These include the use of an integrated Cas9 strain which has the same efficiency as the injection of the recombinant Cas9.<br>Congratulations, New and Montse! <\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/doi.org\/10.1093\/g3journal\/jkad041\">https:\/\/doi.org\/10.1093\/g3journal\/jkad041<\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">Also, check the detailed benchtop protocol here:<\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><blockquote class=\"wp-embedded-content\" data-secret=\"vbtXQyLitD\"><a href=\"http:\/\/livinglight.icfo.eu\/?page_id=2478\">FLint &#8211; Guided Transgene integration using Fluorescence landmark interference<\/a><\/blockquote><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; clip: rect(1px, 1px, 1px, 1px);\" title=\"&#8220;FLint &#8211; Guided Transgene integration using Fluorescence landmark interference&#8221; &#8212; \" src=\"http:\/\/livinglight.icfo.eu\/?page_id=2478&#038;embed=true#?secret=cCHGrUURyE#?secret=vbtXQyLitD\" data-secret=\"vbtXQyLitD\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Happy to announce that Nawaphat&#8217;s paper is peer reviewed and proofed. If you have checked the preprint already, check this one put again &#8211; it has some minor additions. These include the use of an integrated Cas9 strain which has the same efficiency as the injection of the recombinant Cas9.Congratulations, New and Montse! https:\/\/doi.org\/10.1093\/g3journal\/jkad041 Also, &hellip; <a href=\"https:\/\/livinglight.icfo.eu\/?p=2505\" class=\"more-link\">Continue reading <span class=\"screen-reader-text\">FLINTstones &#8211; safe harbor integration of transgenes using CRISPR<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[1],"tags":[],"class_list":["post-2505","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/livinglight.icfo.eu\/index.php?rest_route=\/wp\/v2\/posts\/2505","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/livinglight.icfo.eu\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/livinglight.icfo.eu\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/livinglight.icfo.eu\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/livinglight.icfo.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2505"}],"version-history":[{"count":2,"href":"https:\/\/livinglight.icfo.eu\/index.php?rest_route=\/wp\/v2\/posts\/2505\/revisions"}],"predecessor-version":[{"id":2507,"href":"https:\/\/livinglight.icfo.eu\/index.php?rest_route=\/wp\/v2\/posts\/2505\/revisions\/2507"}],"wp:attachment":[{"href":"https:\/\/livinglight.icfo.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2505"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/livinglight.icfo.eu\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=2505"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/livinglight.icfo.eu\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=2505"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}